LIST OF EXPERIMENTS:

EIGTH SEMESTER:

Bioinformatics:

1.World wide web, internet applications.

2. Introduction of BLASTA/FASTA.

3. Introduction to GCG.

4. NCBI database.

5. Sequence alignment and optimal alignment method.

6. Gene bank sequence database.

7. Multiple sequence alignment.

8. Structure prediction.

9. Genome mapping.

 

SIXTH SEMESTER:

Mass Transfer in Biotechnology

 

1. Determination of diffusion coefficient of an organic vapor (acetone) in air.

2. Study of the drying characteristics of a given material under constant drying conditions and to report equilibrium and critical moisture content.

3. Determination of the mass transfer coefficient for the absorption of water vapor on silica gel.

4. Study the operation of a batch rectification column under constant reflux condition.

5. Study the variation of mass transfer coefficient as a function of flow rate of air for the vaporization of naphthalene in a packed bed.

 

Process Control in Biotechnology

 

1. To determine the valve characteristics of the linear and equal percent valves.

2. To determine the transient response of the first order and second order system.

3. To study the characteristics of flapper nozzle system and to calculate its gain.

4. To study the step response of the first order systems arranged in non interacting and interacting mode and to study its impulse response.

5. To study the advanced control techniques like cascade control, feed forward control, and ratio control using multiprocess trainer.

6. To study the working principle of current to pressure and pressure to current converter using I/P and P/I converter.

7. To study the characteristics of thermistors, thermocouples, and RTD using temperature measurement system.

8. Study of controlling the level using level controller.

9. Study of controlling the temperature using temperature controller.

 

 

 

Industrial Bio-Technology

 

Part A: Industrial biotechnology

1. Production of critic acid from Aspergillus culture.

2. Enzyme immobilization by enzyme entrapment in polyacrylamide gel.

3. Production and estimation of lactic acid and lactose.

4. Cell immobilization with calcium alginate.

5. Preparation of wine.

6. Production and Estimation of alcohol by specific gravity method.

7. Production of enzymes by submerged and solid state fermentation.

 

Part B: Genetic Engineering

1. To study the Restriction mapping

2. Preparation of competent cell for transformation

3. Cacl2 method of transformation

4. DNA cloning and blue white screening of recombinant cells.

 

FOURTH SEMESTER:

Analytical Biotechnology

1. Chromatographic separation of bio-molecules.

2. Determination of adsorption spectrum and extinction coefficient.

3. Demonstration of Gel-permeation chromatography/Ion-exchange/Affinity chromatography.

4. Electrophoresis of Biomolecues i.e. proteins and nucleic acid.

5. Purification of proteins and enzymes by salt precipitation, solvent etc.

6. Turbidometric assay of microbial growth.

Biochemistry Metabolism Laboratory

1. Determination of Michaelis constant of enzymes.

2. Assay of amylase enzyme activity from sweet potato.

3. Assay of phosphatase enzyme activity from potato.

4. Assay of lactate dehydrogenase enzyme activity.

5. Assay of succinate dehydrogenase activity.

6. Assay of alanine aminotransferase activity.

7. Assay of aspartate aminotransferse activity.

8. Isolation and crystallization of enzyme urease from jack beans.

9. Effect of Inhibitor on Enzyme activity

 

Electronics and Instrumentation Laboratory

1. To study the zener diode as a voltage regulator.

2. Study of logic gates.

3. A to D converter

4. D to A converter

5. Study of Bridge rectifier

6. Diac characteristics

7. Study of Thermistor

8. Full wave rectifier

9. Triac characteristics

10. CB, CE amplifier

11. Current to voltage converter

12. Voltage to current converter